mouse il 1 beta quantikine elisa kit Search Results


il1β  (R&D Systems)
96
R&D Systems il1β
Il1β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
il1β - by Bioz Stars, 2026-09
96/100 stars
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immunosorbent assay elisa kits  (R&D Systems)
96
R&D Systems immunosorbent assay elisa kits
TLR4 (toll‐like receptor 4) is indispensable in promoting IL‐1β (interleukin‐1β) secretion from LPS (lipopolysaccharide) + ATP‐activated macrophages. THP‐1 cells were transfected with siRNA (small interfering RNA) or CMV (cytomegalovirus) plasmid to achieve TLR4 knockdown or overexpression, and treated with LPS (1 μg/mL) for 4 h and ATP (3 mmol/L) for 30 min successively. (A) WB (Western blotting) measured TLR4, IκBα, NF‐κB (nuclear factor kappa B) P65, and phosphorylation of IκBα and NF‐κB P65 in macrophages. WB measured pro‐IL‐1β and mature‐IL‐1β levels in macrophages after (B) TLR4 knockdown and after (C) TLR4 overexpression. (D) <t>ELISA</t> (enzyme‐linked <t>immunosorbent</t> assay) measured the protein expression of IL‐1β (interleukin‐1β) and IL‐18 in supernatant ( n = 8). WB was used for measuring NOX2 (NADPH oxidase 2) and NOX4 (NADPH oxidase 4) in ECs (epithelial cells) after (E) TLR4 knockdown and after (F) TLR4 overexpression. Representative fluorescence of mtROS (mitochondrial reactive oxygen species) with MitoSOX in ECs after (G) TLR4 knockdown and after (H) TLR4 overexpression. Data in the histograms were obtained from three independent experiments, and data presentation followed the mean ± SEM (standard error of the mean) format. ANOVA (analysis of variance) was used for the multiple comparisons, and Dunnett's post hoc test (A–C and E–H) or Wilcoxon rank‐sum test (D) was performed subsequently. * p < 0.05 and ** p < 0.01.
Immunosorbent Assay Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+il+1+beta+quantikine+elisa+kit/Mouse+IL-1+beta%2FIL-1F2+Quantikine+ELISA+Kit/pmc12907967-86-15-22
Average 96 stars, based on 1 article reviews
immunosorbent assay elisa kits - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
mouse il 1 beta il 1f2 quantikine hs elisa kit  (R&D Systems)
94
R&D Systems mouse il 1 beta il 1f2 quantikine hs elisa kit
TLR4 (toll‐like receptor 4) is indispensable in promoting IL‐1β (interleukin‐1β) secretion from LPS (lipopolysaccharide) + ATP‐activated macrophages. THP‐1 cells were transfected with siRNA (small interfering RNA) or CMV (cytomegalovirus) plasmid to achieve TLR4 knockdown or overexpression, and treated with LPS (1 μg/mL) for 4 h and ATP (3 mmol/L) for 30 min successively. (A) WB (Western blotting) measured TLR4, IκBα, NF‐κB (nuclear factor kappa B) P65, and phosphorylation of IκBα and NF‐κB P65 in macrophages. WB measured pro‐IL‐1β and mature‐IL‐1β levels in macrophages after (B) TLR4 knockdown and after (C) TLR4 overexpression. (D) <t>ELISA</t> (enzyme‐linked <t>immunosorbent</t> assay) measured the protein expression of IL‐1β (interleukin‐1β) and IL‐18 in supernatant ( n = 8). WB was used for measuring NOX2 (NADPH oxidase 2) and NOX4 (NADPH oxidase 4) in ECs (epithelial cells) after (E) TLR4 knockdown and after (F) TLR4 overexpression. Representative fluorescence of mtROS (mitochondrial reactive oxygen species) with MitoSOX in ECs after (G) TLR4 knockdown and after (H) TLR4 overexpression. Data in the histograms were obtained from three independent experiments, and data presentation followed the mean ± SEM (standard error of the mean) format. ANOVA (analysis of variance) was used for the multiple comparisons, and Dunnett's post hoc test (A–C and E–H) or Wilcoxon rank‐sum test (D) was performed subsequently. * p < 0.05 and ** p < 0.01.
Mouse Il 1 Beta Il 1f2 Quantikine Hs Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+il+1+beta+quantikine+elisa+kit/Mouse+IL-1+beta%2FIL-1F2+Quantikine+HS+ELISA+Kit/10__1016_slash_j__heliyon__2025__e42976-53-92-99
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mouse il 1 beta il 1f2 quantikine hs elisa kit - by Bioz Stars, 2026-09
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il 1β mhslb00  (R&D Systems)
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R&D Systems il 1β mhslb00
TLR4 (toll‐like receptor 4) is indispensable in promoting IL‐1β (interleukin‐1β) secretion from LPS (lipopolysaccharide) + ATP‐activated macrophages. THP‐1 cells were transfected with siRNA (small interfering RNA) or CMV (cytomegalovirus) plasmid to achieve TLR4 knockdown or overexpression, and treated with LPS (1 μg/mL) for 4 h and ATP (3 mmol/L) for 30 min successively. (A) WB (Western blotting) measured TLR4, IκBα, NF‐κB (nuclear factor kappa B) P65, and phosphorylation of IκBα and NF‐κB P65 in macrophages. WB measured pro‐IL‐1β and mature‐IL‐1β levels in macrophages after (B) TLR4 knockdown and after (C) TLR4 overexpression. (D) <t>ELISA</t> (enzyme‐linked <t>immunosorbent</t> assay) measured the protein expression of IL‐1β (interleukin‐1β) and IL‐18 in supernatant ( n = 8). WB was used for measuring NOX2 (NADPH oxidase 2) and NOX4 (NADPH oxidase 4) in ECs (epithelial cells) after (E) TLR4 knockdown and after (F) TLR4 overexpression. Representative fluorescence of mtROS (mitochondrial reactive oxygen species) with MitoSOX in ECs after (G) TLR4 knockdown and after (H) TLR4 overexpression. Data in the histograms were obtained from three independent experiments, and data presentation followed the mean ± SEM (standard error of the mean) format. ANOVA (analysis of variance) was used for the multiple comparisons, and Dunnett's post hoc test (A–C and E–H) or Wilcoxon rank‐sum test (D) was performed subsequently. * p < 0.05 and ** p < 0.01.
Il 1β Mhslb00, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+il+1+beta+quantikine+elisa+kit/Mouse+IL-1+beta%2FIL-1F2+Quantikine+HS+ELISA+Kit/pmc08437825-226-21-24
Average 94 stars, based on 1 article reviews
il 1β mhslb00 - by Bioz Stars, 2026-09
94/100 stars
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Image Search Results


TLR4 (toll‐like receptor 4) is indispensable in promoting IL‐1β (interleukin‐1β) secretion from LPS (lipopolysaccharide) + ATP‐activated macrophages. THP‐1 cells were transfected with siRNA (small interfering RNA) or CMV (cytomegalovirus) plasmid to achieve TLR4 knockdown or overexpression, and treated with LPS (1 μg/mL) for 4 h and ATP (3 mmol/L) for 30 min successively. (A) WB (Western blotting) measured TLR4, IκBα, NF‐κB (nuclear factor kappa B) P65, and phosphorylation of IκBα and NF‐κB P65 in macrophages. WB measured pro‐IL‐1β and mature‐IL‐1β levels in macrophages after (B) TLR4 knockdown and after (C) TLR4 overexpression. (D) ELISA (enzyme‐linked immunosorbent assay) measured the protein expression of IL‐1β (interleukin‐1β) and IL‐18 in supernatant ( n = 8). WB was used for measuring NOX2 (NADPH oxidase 2) and NOX4 (NADPH oxidase 4) in ECs (epithelial cells) after (E) TLR4 knockdown and after (F) TLR4 overexpression. Representative fluorescence of mtROS (mitochondrial reactive oxygen species) with MitoSOX in ECs after (G) TLR4 knockdown and after (H) TLR4 overexpression. Data in the histograms were obtained from three independent experiments, and data presentation followed the mean ± SEM (standard error of the mean) format. ANOVA (analysis of variance) was used for the multiple comparisons, and Dunnett's post hoc test (A–C and E–H) or Wilcoxon rank‐sum test (D) was performed subsequently. * p < 0.05 and ** p < 0.01.

Journal: Animal Models and Experimental Medicine

Article Title: Polydatin alleviates mitochondrial damage and apoptosis of lung epithelial cells by inhibiting toll‐like receptor 4‐dependent macrophage activation in asthma

doi: 10.1002/ame2.70100

Figure Lengend Snippet: TLR4 (toll‐like receptor 4) is indispensable in promoting IL‐1β (interleukin‐1β) secretion from LPS (lipopolysaccharide) + ATP‐activated macrophages. THP‐1 cells were transfected with siRNA (small interfering RNA) or CMV (cytomegalovirus) plasmid to achieve TLR4 knockdown or overexpression, and treated with LPS (1 μg/mL) for 4 h and ATP (3 mmol/L) for 30 min successively. (A) WB (Western blotting) measured TLR4, IκBα, NF‐κB (nuclear factor kappa B) P65, and phosphorylation of IκBα and NF‐κB P65 in macrophages. WB measured pro‐IL‐1β and mature‐IL‐1β levels in macrophages after (B) TLR4 knockdown and after (C) TLR4 overexpression. (D) ELISA (enzyme‐linked immunosorbent assay) measured the protein expression of IL‐1β (interleukin‐1β) and IL‐18 in supernatant ( n = 8). WB was used for measuring NOX2 (NADPH oxidase 2) and NOX4 (NADPH oxidase 4) in ECs (epithelial cells) after (E) TLR4 knockdown and after (F) TLR4 overexpression. Representative fluorescence of mtROS (mitochondrial reactive oxygen species) with MitoSOX in ECs after (G) TLR4 knockdown and after (H) TLR4 overexpression. Data in the histograms were obtained from three independent experiments, and data presentation followed the mean ± SEM (standard error of the mean) format. ANOVA (analysis of variance) was used for the multiple comparisons, and Dunnett's post hoc test (A–C and E–H) or Wilcoxon rank‐sum test (D) was performed subsequently. * p < 0.05 and ** p < 0.01.

Article Snippet: Concentrations of IL‐1β and IL‐18 in BALF and culture supernatants were quantified using commercial enzyme‐linked immunosorbent assay (ELISA) kits (MLB00C and DY7625‐05, R&D Systems, Minneapolis) following previously established methodologies.

Techniques: Transfection, Small Interfering RNA, Plasmid Preparation, Knockdown, Over Expression, Western Blot, Phospho-proteomics, Enzyme-linked Immunosorbent Assay, Expressing, Fluorescence

Polydatin relieves ROS (reactive oxygen species) and inflammation in asthmatic mice. To investigate asthma pathology, we developed an asthma mouse model via sensitization of BALB/c mice with ovalbumin (OVA). After initial OVA exposure, animals received intraperitoneal injections of polydatin prior to subsequent aerosolized OVA challenges. (A) Structural representation of polydatin. (B) Experimental protocol diagram illustrating timeline and treatment schedules. (C) Lung resistance alterations in response to methacholine stimulation ( n = 8 per group). (D) Quantification of eosinophils (identified as CD45.2 + Siglec‐F + cells) within bronchoalveolar lavage fluid (BALF) using flow cytometry. (E) Representative histological lung sections stained with hematoxylin–eosin (HE) and periodic acid–Schiff (PAS). (F) Measurement of cytokine concentrations in BALF using ELISA (enzyme‐linked immunosorbent assay) assays ( n = 8 per group). (G) Fluorescent microscopic images demonstrating ROS (reactive oxygen species) production via dihydroethidium (DHE) staining in lung tissues. (H) WB (Western blotting) quantification of NOX2 (NADPH oxidase 2) and NOX4 (NADPH oxidase 4) protein expression in pulmonary tissues. (I) Identification of apoptotic cells using TUNEL (terminal deoxynucleotidyl transferase dUTP nick‐end labeling) staining in lung sections with corresponding quantitative analysis of positive cells ( n = 3). (J) WB detection of apoptosis‐related proteins within lung homogenates. (K) Immunohistochemical staining depicting the expression and localization of cleaved caspase‐3 and cleaved caspase‐9 proteins ( n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001.

Journal: Animal Models and Experimental Medicine

Article Title: Polydatin alleviates mitochondrial damage and apoptosis of lung epithelial cells by inhibiting toll‐like receptor 4‐dependent macrophage activation in asthma

doi: 10.1002/ame2.70100

Figure Lengend Snippet: Polydatin relieves ROS (reactive oxygen species) and inflammation in asthmatic mice. To investigate asthma pathology, we developed an asthma mouse model via sensitization of BALB/c mice with ovalbumin (OVA). After initial OVA exposure, animals received intraperitoneal injections of polydatin prior to subsequent aerosolized OVA challenges. (A) Structural representation of polydatin. (B) Experimental protocol diagram illustrating timeline and treatment schedules. (C) Lung resistance alterations in response to methacholine stimulation ( n = 8 per group). (D) Quantification of eosinophils (identified as CD45.2 + Siglec‐F + cells) within bronchoalveolar lavage fluid (BALF) using flow cytometry. (E) Representative histological lung sections stained with hematoxylin–eosin (HE) and periodic acid–Schiff (PAS). (F) Measurement of cytokine concentrations in BALF using ELISA (enzyme‐linked immunosorbent assay) assays ( n = 8 per group). (G) Fluorescent microscopic images demonstrating ROS (reactive oxygen species) production via dihydroethidium (DHE) staining in lung tissues. (H) WB (Western blotting) quantification of NOX2 (NADPH oxidase 2) and NOX4 (NADPH oxidase 4) protein expression in pulmonary tissues. (I) Identification of apoptotic cells using TUNEL (terminal deoxynucleotidyl transferase dUTP nick‐end labeling) staining in lung sections with corresponding quantitative analysis of positive cells ( n = 3). (J) WB detection of apoptosis‐related proteins within lung homogenates. (K) Immunohistochemical staining depicting the expression and localization of cleaved caspase‐3 and cleaved caspase‐9 proteins ( n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Concentrations of IL‐1β and IL‐18 in BALF and culture supernatants were quantified using commercial enzyme‐linked immunosorbent assay (ELISA) kits (MLB00C and DY7625‐05, R&D Systems, Minneapolis) following previously established methodologies.

Techniques: Flow Cytometry, Staining, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, TUNEL Assay, Immunohistochemical staining

Polydatin suppresses TLR4 (toll‐like receptor 4)/P2X7R/NLRP3 (NOD‐like receptor protein) activation in OVA (ovalbumin)–induced asthma. WB (Western blotting) measured (A) TLR4, IκBα, NF‐κB (nuclear factor kappa B) P65, and phosphorylation of IκBα and NF‐κB P65, and (B) P2X7R, NLRP3, ASC, and caspase‐1 in lung tissues. (C) Co‐expression of F4/80 (green) and (D) P2X7R (green) with TLR4 (red) in mouse lung tissues was assessed via immunofluorescence staining combined ( n = 3). (E) ELISA (enzyme‐linked immunosorbent assay) and real‐time PCR (polymerase chain reaction) were used to measure IL‐1β (interleukin‐1β) and IL‐18 protein levels in BALF (bronchoalveolar lavage fluid) and mRNA (messenger RNA) expression ( n = 8), and Dunnett's post hoc test (A–D) or Wilcoxon rank‐sum test (E) was performed subsequently. * p < 0.05 and ** p < 0.01.

Journal: Animal Models and Experimental Medicine

Article Title: Polydatin alleviates mitochondrial damage and apoptosis of lung epithelial cells by inhibiting toll‐like receptor 4‐dependent macrophage activation in asthma

doi: 10.1002/ame2.70100

Figure Lengend Snippet: Polydatin suppresses TLR4 (toll‐like receptor 4)/P2X7R/NLRP3 (NOD‐like receptor protein) activation in OVA (ovalbumin)–induced asthma. WB (Western blotting) measured (A) TLR4, IκBα, NF‐κB (nuclear factor kappa B) P65, and phosphorylation of IκBα and NF‐κB P65, and (B) P2X7R, NLRP3, ASC, and caspase‐1 in lung tissues. (C) Co‐expression of F4/80 (green) and (D) P2X7R (green) with TLR4 (red) in mouse lung tissues was assessed via immunofluorescence staining combined ( n = 3). (E) ELISA (enzyme‐linked immunosorbent assay) and real‐time PCR (polymerase chain reaction) were used to measure IL‐1β (interleukin‐1β) and IL‐18 protein levels in BALF (bronchoalveolar lavage fluid) and mRNA (messenger RNA) expression ( n = 8), and Dunnett's post hoc test (A–D) or Wilcoxon rank‐sum test (E) was performed subsequently. * p < 0.05 and ** p < 0.01.

Article Snippet: Concentrations of IL‐1β and IL‐18 in BALF and culture supernatants were quantified using commercial enzyme‐linked immunosorbent assay (ELISA) kits (MLB00C and DY7625‐05, R&D Systems, Minneapolis) following previously established methodologies.

Techniques: Activation Assay, Western Blot, Phospho-proteomics, Expressing, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, RNA Expression

Polydatin inhibits IL‐1β (interleukin‐1β) and IL‐18 by blocking the TLR4 (toll‐like receptor 4)/P2X7R pathway in macrophages. (A) Intracellular calcium concentrations were analyzed utilizing Fluo 3‐AM fluorescent staining ( n = 3). (B) WB (Western blotting) was performed to quantify the levels of TLR4, IκBα, NF‐κB (nuclear factor kappa B) p65, phosphorylated forms of IκBα and NF‐κB p65, P2X7R, NLRP3 (NOD‐like receptor protein) inflammasome components, IL‐1β, and IL‐18 ( n = 3). (C) Macrophage‐derived protein secretion levels of IL‐1β and IL‐18 were evaluated using ELISA (enzyme‐linked immunosorbent assay). (A and B) or Wilcoxon rank‐sum testing (C). * p < 0.05 and ** p < 0.01.

Journal: Animal Models and Experimental Medicine

Article Title: Polydatin alleviates mitochondrial damage and apoptosis of lung epithelial cells by inhibiting toll‐like receptor 4‐dependent macrophage activation in asthma

doi: 10.1002/ame2.70100

Figure Lengend Snippet: Polydatin inhibits IL‐1β (interleukin‐1β) and IL‐18 by blocking the TLR4 (toll‐like receptor 4)/P2X7R pathway in macrophages. (A) Intracellular calcium concentrations were analyzed utilizing Fluo 3‐AM fluorescent staining ( n = 3). (B) WB (Western blotting) was performed to quantify the levels of TLR4, IκBα, NF‐κB (nuclear factor kappa B) p65, phosphorylated forms of IκBα and NF‐κB p65, P2X7R, NLRP3 (NOD‐like receptor protein) inflammasome components, IL‐1β, and IL‐18 ( n = 3). (C) Macrophage‐derived protein secretion levels of IL‐1β and IL‐18 were evaluated using ELISA (enzyme‐linked immunosorbent assay). (A and B) or Wilcoxon rank‐sum testing (C). * p < 0.05 and ** p < 0.01.

Article Snippet: Concentrations of IL‐1β and IL‐18 in BALF and culture supernatants were quantified using commercial enzyme‐linked immunosorbent assay (ELISA) kits (MLB00C and DY7625‐05, R&D Systems, Minneapolis) following previously established methodologies.

Techniques: Blocking Assay, Staining, Western Blot, Derivative Assay, Enzyme-linked Immunosorbent Assay